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The advent of the PCR has resulted in the development of a large number of molecular techniques, which offer an effective alternative to the hybridization methods of RFLP analysis.
To evaluate RFLP band length differences across gels could be difficult, and the method requires much larger quantities of DNA than a PCR-based screening method.
Examination of viral isolates using restriction fragment length polymorphism of polymerase chain reaction (PCR-RFLP) from individual ILTV genes has suggested that some of these outbreaks were caused ...
Interspecific and intraspecific variation in the internal transcribed spacer (ITS) region of rDNA of ectomycorrhizal fungi of 44 species in 17 genera were examined using the restriction fragment ...
Another team member, Sastry, added that the team had initially attempted to use microsatellite markers for species identification but found the PCR-RFLP method to be far more reliable.